Fluorescence Reader

• Genotyping of allele conditions extremely simple and fast
• Combines advantages of rapidPCR and simple endpoint detection
• Integrated genotyping software ASpect SNP
• Multi-component analysis
• 96 samples in parallel within less than 60 minutes
• Risk of contamination is significantly restricted
• Alternative to other expensive SNP detection systems
• Can be equipped with up to 6 excitation and emission filters of choice
• Applicable as simple PCR endpoint detector
• Ideal for routine diagnostic
• Read-out of 96 wells within 4 sec (independent of the number of dyes)

Measuring method

The analysis is based on the combination of rapidPCR and subsequent endpoint fluorescence detection. The test principle is building on robust homogeneous exonuclease assay. After execution of the exonuclease assays the detection of the fluorescence signals takes place in form of an endpoint determination (not in real-time).
Basis for the final analysis of the allele conditions is the integrated genotyping software ASpect SNP. With this software the analytic evaluation of measured endpoint fluorescence of both probes takes place by means of a multi-component analysis. This analysis makes the accurate determination of the available allele condition possible. This new kind of genotyping is extreme fast and permits the measurement of up to 96 samples in parallel within less than 60 minutes.

Prepared for the future

To fit different demands of applications the SNPtower can be equipped with up to 6 excitation and emission filters of choice. These filters are easily interchangeable by the user what keeps the system open for future adaptations or changes. Therefore the instrument is ideally suited for multiplex applications and covers most currently available dyestuffs.

Fast detection of PCR products

In addition to SNP diagnostics the SNPtower can be also used as fluorescence reader for easy determination of PCR products. The risk of contamination is significantly restricted due to determination of PCR products without opening the reaction vessel. Particularly for diagnostic applications, simple yes/ no statements are frequently sufficient, e.g. in demonstrating the simple presence of a microorganism in a clinical sample as evidence of an infection.

ASpect SNP – simple and clear

Intuitive, fast and easy operation are hallmarks of the SNPtower software. It not only controls the PCR reaction and the following measurement processes, but also permits an extensive, GLP-complaint evaluation, an user management and also allows user rights to be assigned.

• Configurable graphic representations of all recorded data
• Freely selectable colour channels and linkage with individual evaluation criteria, storage for re-use as user-defined templates
• SNP detection evaluation algorithms
• Bilingualism at the press of a button
• Context-related online help
• User management and audit trail

NOTE: the SNPtower is not applicable for real-time PCR but only for endpoint detection by means of detection before and after the PCR run.

• Fast PCR fluorescence detection
• Ideal for diagnostic applications (yes/no statements)
• SNP detection evaluation algorithms available
• Cost-effective alternative to expensive real-time systems
• Significantly reduced risk of contamination
• Up to 4 different fluorescence filters selectable
• Many times faster than gel electrophoresis
• The ideal supplement for existing thermal cyclers
• Simple & clear software
• Configurable graphic representations of all recorded data
• Individual evaluation criteria, storage for re-use as user-defined templates
• Bilingualism at the press of a button
• Context-related online help
• User management and audit trail
  

Fast fluorescence detection of PCR products

The polymerase chain reaction (PCR) is one of the absolutely key technologies in modern biological research and molecular diagnostics. Its absence in any biological laboratory is unimaginable. The demands placed on apparatus in the PCR laboratory to guarantee reliable analyses at all times are on the increase, along with the number of PCR applications in routine laboratory work. The type of detection therefore has a crucial influence on the reliability and interpretation of PCR results. The agarose gel used in electrophoresis detection of PCR products is, for example, especially prone to the latent risk of contamination of samples and the laboratory and consequently false-positive results.

High speeds and flexibility

PCR end-point detection offers further essential advantages.The introduction of rapid thermal cyclers, such as the SpeedCycler, allows typical amplifications to be performed in down to 8 minutes. Traditional gel electrophoresis with a running time of 30 — 45 minutes plus preparation therefore represents a time restraint. However, the read-out of a 96 well PCR microplate with SpeedScan is completed after 50 seconds per color channel. SpeedScan can record up to 4 different fluorescence filters, which makes it ideally suited for multiplex applications. Naturally  the fluorescence filter is free to use and exchange.

ASpect FA – simple and clear

Intuitive, fast and easy operation are hallmarks of the SpeedScan software. It not only controls the measurement processes,but also permits an extensive, GLP-complaint evaluation, an user management and also allows user rights to be assigned.

Available accessories for the SpeedScan

• Tray for standard microplates
• Adapter for measurement of 2x 36 well microplates LP
• Adapter for measurement of 1x 96 well microplates LP
• Adapter for measurement of 0.2 ml standard tubes and strips
• Calibration plate - adapter plate for the SpeedScan validation