Automatic  Purfication

Folder InnuPureC96

Fast and efficient high-throughput nucleic acid extraction

 

The InnuPure® C96 allows a fast and fully automated nucleic acid extraction from complex starting materials in 96-well standard format. The InnuPure® C96 extraction system is founded on the proven principles of liquid handling and purification based on magnetic particle separation. Therefore high yields and excellent purities are achieved. The purification of DNA and/or RNA is one of the most common methods for sample preparation and thus constitutes a standard in molecular biology and medical diagnostics.

The built-in 96 well precision pipetting head with 96 simultaneously operating channels and an established tip sealing principle is well suited for complex purification processes with high sample throughput. It also ensures excellent and reproducible results. The automated extraction process also allows a flexible time management which enables to plan and prepare subsequent experiments comprehensively.

 
  • Fully automated nucleic acid extraction based on proven magnetic particle separation
  • Preparation of up to 96 samples in parallel
  • Preprogrammed extraction protocols for optimal reproducibility
  • Adjustable elution volumes
  • Reagent plasticware filled up automatically with all necessary solutions
  • Minimum number of manual steps
  • Tight desktop device for any lab bench
  • Optimized lysis by using a thermal shaker with up to 3,000 rpm and 99°C
  • Minimized contamination and easy decontamination due to an integrated UV lamp and a HEPA filter
  • Highly flexible system for a wide variety of starting materials and volumes
  • Ready-to-use purification kits for easy handling and for the extraction of high quality nucleic acids

 

Fast preparation with minimal effort

After the InnuPure® C96 has been equipped with all necessary plastic materials and buffer reservoirs , the automatic filling routine is started. Then the starting material is introduced into the isolation process. The subsequent purification process and the supply or discharge of the necessary reagents take place along a linear distance with 5 function positions. The plate transport system consisting of a three position wagon guarantees a high level of flexibility and speed. The nucleic acids, bound to magnetic particles, are collected at the bottom of the wells and, depending on the protocol, resolubilized by up and down pipetting in an optimized process. An integrated lifter enables microplates to be moved between the positions of the wagon. Using an automated, optionally available thermal shaker - the BioShake 3000-T elm - necessary heating steps can be performed without any manual intervention. Therefore the lysis efficiency can be set optimally while reducing the lysis duration at the same time.

In addition, the InnuPure® C96 provides maximum protection against possible contamination, which can be successfully prevented with the help of the innovative extraction principle. An integrated UV lamp, an optional HEPA filter with a fan and the closed working area make the contamination protection of the extraction system, the individual samples and the isolated nucleic acids complete.

 

High operating comfort for every routine application

High quality robot technology with a very user-friendly software make the InnuPure® C96 to an attractive extraction system, both in research institutions and for its use in routine applications. The effective and flexible software allows preprogrammed standard methods to be loaded, protocols to be configured easily and to be adjusted to customer-specific applications. The clear user interface ensures current experiments to be easily understood by providing schematic and graphical representations, detailed user instructions and displaying the active extraction step in real-time.

 

Flexible, optimized kits for optimal nucleic acid purification

For the InnuPure® C96 a variety of different DNA/RNA extraction kits is available. Based on the proven separation of nucleic acids bound to magnetic particles, excellent results with high purity and yield are guaranteed. This ensures the final product to be free of proteins, nucleases and other contaminants and to be used immediately for subsequent applications. The whole system makes sure that time is saved significantly and manual interventions are reduced to an absolute minimum. Pipetting, mixing and heating steps including in the routine are all operated by the extraction automat.

 
FolderInnuPureC16

The InnuPure C16® is a new, flexible and efficient extraction system for fully automated isolation and purification of nucleic acids. The system, which was developed and manufactured in Germany, is designed for small and medium sample throughput and can process a wide range of starting materials. The system combines a unique liquid handling method with an extremely fast walk-away principle.

Labor-intensive sample lysis steps are no longer necessary, as they are now incorporated into the automated extraction process in keeping with the starting material. The nucleic acids to be isolated are then adsorbed onto magnetic or paramagnetic particles whose surfaces have been specially adapted for this purpose. The required extraction chemistry has been optimized for the application at hand, allowing users to isolate high yields of very pure nucleic acids.

 
  • Flexible and efficient extraction system soon
  • Completely automated and compact
  • Up to sixteen samples in parallel
  • Isolation of very pure nucleic acids
  • Suitable for a wide variety of starting materials, including forensic samples
  • Pre-programmed protocols
  • Adsorption of the isolated material onto magnetic or paramagnetic particles
  • Automatic transfer of eluates into Elution Tubes with caps
  • Easy and convenient to use thanks to the portable HID-Pro 320 user interface
  • No cross-contamination
  • Highly reproducible
  • Fast, reliable and efficient

 

The fully automatic magnetic particle separation process is carried out in the wells of the plastic extraction vessels. After the starting material has been introduced into the isolation process, the necessary reagents are pipetted to the sample and then automatically removed by pipette tip.

Once the nucleic acids have been bound to magnetic particles, they collect at the bottom of the wells and, depending on the routine in use, are resolubilized by pipetting them in and out in an optimized process. Finally, the DNA and/or RNA are eluted into separate, capped Elution Tubes for direct storage or other applications. The extraction principle also efficiently prevents the cross contamination that often occurs in vacuum-based purification methods. In addition, the InnuPure C16 is equipped with pre-installed application protocols in order to avoid time-consuming programming. The high flexibility of the system allows users to isolate DNA or RNA from up to 16 samples in parallel.

 

HID-Pro 320 for fast and easy operation

Users operate the InnuPure C16® from a flexible, portable HID-Pro 320 unit with the large 5.7" touchscreen. Because this PC-based system operates in a Windows CE environment, users have typical Windows functions and a clearly structured menu on the interface, making the entire system a stand-alone device. The real-time display allows scientists to check the status of the current extraction and follow each routine clearly and continuously. In addition, the USB interface makes it easy to update software and upload new isolation protocols.

 

Extremely easy handling thanks to optimized extraction kits

Extraction kits adapted to the InnuPure C16® allow users to process forensic samples and isolate genomic DNA, cellular RNA, and viral or bacterial nucleic acids. These kits are ideal automation tools for efficiently isolating high-quality nucleic acids with no contamination. All purification kits are ready for use and have been optimized for different starting materials and quantities.

Fully pre-filled Reagent Strips and Plates minimize manual steps and save an impressive amount of time. In addition, the intelligent sample tray allows users to process individual samples. Therefore the positions of the deep-well Reagent Plates can be assembled with an additional adapter: a process that can be performed in just one easy step. This makes it possible to adapt up to 4 prefilled Reagent Strips for individual sample handling and, as such, the InnuPure C16® can be easily adapted to handle a quantity from 1 to 16 samples.

 

 
td_InnuPureC12_e InnuPure_C12_flyer_e

Extraction system

 

The InnuPure® C12 is a new, flexible and efficient extraction system for the completely automated isolation and purification of nucleic acids.

 

The system, which is developed and produced in Germany, is designed for small and medium sample throughput. A wide range of starting materials can be processed. The system combines a unique way of performing liquid handling steps with an extremely fast walk-away principle.

 

The InnuPure® C12 is equipped with pre-installed application protocols to eliminate time-consuming programming. The high degree of flexibility of InnuPure® C12 allows the isolation of DNA or RNA from up to twelve samples in parallel.

 
  • Nucleic acid purification robot
  • Fast, reliable and efficient
  • High operating convenience
  • Flexible and efficient extraction system
  • For numerous types of starting materials
  • Completely automated and compact
  • Very simple operation
  • Pre-programmed protocols
  • Up to twelve samples in parallel
  • Adsorption of the isolated material to magnetic or paramagnetic particles
  • Isolation of very pure nucleic acids
  • Automatic transfer of eluates into Eluation Tubes with lids
  • No cross-contamination
  • Separate Waste box, easy to clean
  • High reproducibility
  • Very rapid extraction of genomic DNA, cellular RNA, viral and bacterial nucleic acids 
  • Processing of forensic samples

 

Unique fast walk – away principle

The work intensive steps for sample lysis, that were often necessary up to now are now incorporated into the automated extraction process, depending on the starting material.
The nucleic acids to be isolated are adsorbed to surface functionalized magnetic or paramagnetic particles. The required extraction chemistry is optimally adapted to the application at hand and facilitates the isolation of very pure nucleic acids with excellent yields.

The extraction principle effectively prevents cross-contamination, a problem that frequently occurs in vacuum-based extraction methods. A separate and removable waste box reduces the contamination risk furthermore. The isolated nucleic acids can be used immediately afterwards for downstream applications. Extraction kits optimized for InnuPure® C12 are available for the isolation of genomic DNA, cellular RNA, viral or bacterial nucleic acids, as well as the processing of forensic samples. The extraction process requires between 20 and 40 minutes, depending upon application.

 

Ready-to-use Kits

The innuPREP Kits – IP are delivered ready-to-use with all necessary solutions and plastics for minimal handling steps. The pre-filled, sealed strips facilitate a convenient and fast preparation of the sample tray and thus the nucleic acid isolation.
Additional the piercing function of the InnuPure® C12 renders the removal of the sealing foil necessary. Finally the eluate is transferred to an Elution Tube and ready-to-use for any down stream application.